![]() piperita at a concentration of 35 mg/L (value determined by the acute toxicity trial), and two controls, one positive and a negative. A three-replicate treatment was carried out, where the fish were submitted to 60-min baths with essential oil of M. To test the efficacy of the essential oil against the parasite in the live host, it was performed an in vivo trial with 27 naturally infected juvenile Nile Tilapia. After the acute toxicity trial, histological analyzes were performed on the gills of specimens submitted to acute toxicity concentrations and controls. It was tested the lethal concentration for 48 h (LC50-48 h), and during this period we recorded the behavior and mortality of individuals. In the acute toxicity trial in fingerlings, seven oil concentrations (20, 25, 30, 35, 40, 45 and 50 mg/L) were evaluated with two controls, one positive (water) and one negative control (water with addition of 35 mL/L of absolute ethyl alcohol-99.8% PA). This study determined the safe dosage of Mentha piperita essential oil in Oreochromis niloticus and tested its effectiveness against gill monogeneans.
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